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Continuous Fluorescence Assays for Reactions Involving Adenine
5′-Methylthioadenosine phosphorylase (MTAP) and 5′-methylthioadenosine nucleosidase (MTAN) catalyze the phosphorolysis and hydrolysis of 5′-methylthioadenosine (MTA), respectively. Both enzymes have low K(M) values for their substrates. Kinetic assays for these enzymes are challenging, as the ultrav...
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| Publicado no: | Anal Chem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5434977/ https://ncbi.nlm.nih.gov/pubmed/27779859 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.6b03621 |
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