A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria

The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available...

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Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Front Microbiol
Päätekijät: Mugweru, Julius, Makafe, Gaelle, Cao, Yuanyuan, Zhang, Yang, Wang, Bangxing, Huang, Shaobo, Njire, Moses, Chhotaray, Chiranjibi, Tan, Yaoju, Li, Xinjie, Liu, Jianxiong, Tan, Shouyong, Deng, Jiaoyu, Zhang, Tianyu
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Frontiers Media S.A. 2017
Aiheet:
Microbiology
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5364183/
https://ncbi.nlm.nih.gov/pubmed/28392781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.00468
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