A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria

The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available...

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Bibliografski detalji
Izdano u:Front Microbiol
Glavni autori: Mugweru, Julius, Makafe, Gaelle, Cao, Yuanyuan, Zhang, Yang, Wang, Bangxing, Huang, Shaobo, Njire, Moses, Chhotaray, Chiranjibi, Tan, Yaoju, Li, Xinjie, Liu, Jianxiong, Tan, Shouyong, Deng, Jiaoyu, Zhang, Tianyu
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2017
Teme:
Microbiology
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5364183/
https://ncbi.nlm.nih.gov/pubmed/28392781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.00468
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