A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria

The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available...

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Vydáno v:Front Microbiol
Hlavní autoři: Mugweru, Julius, Makafe, Gaelle, Cao, Yuanyuan, Zhang, Yang, Wang, Bangxing, Huang, Shaobo, Njire, Moses, Chhotaray, Chiranjibi, Tan, Yaoju, Li, Xinjie, Liu, Jianxiong, Tan, Shouyong, Deng, Jiaoyu, Zhang, Tianyu
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2017
Témata:
Microbiology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5364183/
https://ncbi.nlm.nih.gov/pubmed/28392781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.00468
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