A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria

The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available...

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Detalles Bibliográficos
Publicado en:Front Microbiol
Autores principales: Mugweru, Julius, Makafe, Gaelle, Cao, Yuanyuan, Zhang, Yang, Wang, Bangxing, Huang, Shaobo, Njire, Moses, Chhotaray, Chiranjibi, Tan, Yaoju, Li, Xinjie, Liu, Jianxiong, Tan, Shouyong, Deng, Jiaoyu, Zhang, Tianyu
Formato: Artigo
Lenguaje:Inglês
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5364183/
https://ncbi.nlm.nih.gov/pubmed/28392781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.00468
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