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Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR

PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular ‘batch-stamps’ that effe...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Miner, Brooks E., Stöger, Reinhard J., Burden, Alice F., Laird, Charles D., Hansen, R. Scott
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2004
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC521679/
https://ncbi.nlm.nih.gov/pubmed/15459281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh132
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