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Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR
PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular ‘batch-stamps’ that effe...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC521679/ https://ncbi.nlm.nih.gov/pubmed/15459281 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh132 |
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