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Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR

PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular ‘batch-stamps’ that effe...

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Bibliografiska uppgifter
Huvudupphovsmän: Miner, Brooks E., Stöger, Reinhard J., Burden, Alice F., Laird, Charles D., Hansen, R. Scott
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2004
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC521679/
https://ncbi.nlm.nih.gov/pubmed/15459281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh132
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