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Control of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA
In this study, we adapted the well known uracil DNA glycosylase (UNG) carry-over prevention system for PCR, and applied it to the analysis of DNA methylation based on sodium bisulfite conversion. As sodium bisulfite treatment converts unmethylated cytosine bases into uracil residues, bisulfite treat...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2007
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1747185/ https://ncbi.nlm.nih.gov/pubmed/17135186 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl955 |
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