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Control of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA

In this study, we adapted the well known uracil DNA glycosylase (UNG) carry-over prevention system for PCR, and applied it to the analysis of DNA methylation based on sodium bisulfite conversion. As sodium bisulfite treatment converts unmethylated cytosine bases into uracil residues, bisulfite treat...

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Detaylı Bibliyografya
Asıl Yazarlar: Tetzner, Reimo, Dietrich, Dimo, Distler, Juergen
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 2007
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1747185/
https://ncbi.nlm.nih.gov/pubmed/17135186
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl955
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