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Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR
We report a novel method for rapid quantification of the degree of DNA methylation of a specific gene. Our method combined bisulfite-mediated PCR and quantification of deoxyribonucleoside monophosphate (dNMP) contents in the PCR product through capillary electrophoresis. A specific bisulfite-PCR pro...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1458279/ https://ncbi.nlm.nih.gov/pubmed/16679448 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl257 |
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