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Accurate quantification of supercoiled DNA by digital PCR

Digital PCR (dPCR) as an enumeration-based quantification method is capable of quantifying the DNA copy number without the help of standards. However, it can generate false results when the PCR conditions are not optimized. A recent international comparison (CCQM P154) showed that most laboratories...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Dong, Lianhua, Yoo, Hee-Bong, Wang, Jing, Park, Sang-Ryoul
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 2016
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4827127/
https://ncbi.nlm.nih.gov/pubmed/27063649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep24230
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