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PCR hot start using primers with the structure of molecular beacons (hairpin-like structure)

A new technique of PCR hot start using oligonucleotide primers with a stem–loop structure is developed here. The molecular beacon oligonucleotide structure without any chromophore addition to the ends was used. The 3′-end sequence of the primers was complementary to the target and five or six nucleo...

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Bibliografische gegevens
Hoofdauteurs: Kaboev, O. K., Luchkina, L. A., Tret’iakov, A. N., Bahrmand, A. R.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2000
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC113163/
https://ncbi.nlm.nih.gov/pubmed/11058144
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