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PCR hot start using primers with the structure of molecular beacons (hairpin-like structure)

A new technique of PCR hot start using oligonucleotide primers with a stem–loop structure is developed here. The molecular beacon oligonucleotide structure without any chromophore addition to the ends was used. The 3′-end sequence of the primers was complementary to the target and five or six nucleo...

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Sábháilte in:
Sonraí bibleagrafaíochta
Foilsithe in:Nucleic Acids Res
Príomhchruthaitheoirí: Kaboev, O. K., Luchkina, L. A., Tret’iakov, A. N., Bahrmand, A. R.
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Oxford University Press 2000
Ábhair:
Rochtain ar líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113163/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11058144/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.21.e94
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