PCR hot start using primers with the structure of molecular beacons (hairpin-like structure)
A new technique of PCR hot start using oligonucleotide primers with a stem–loop structure is developed here. The molecular beacon oligonucleotide structure without any chromophore addition to the ends was used. The 3′-end sequence of the primers was complementary to the target and five or six nucleo...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113163/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11058144/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.21.e94 |
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