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Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR

PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular ‘batch-stamps’ that effe...

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Bibliografske podrobnosti
Main Authors: Miner, Brooks E., Stöger, Reinhard J., Burden, Alice F., Laird, Charles D., Hansen, R. Scott
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2004
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC521679/
https://ncbi.nlm.nih.gov/pubmed/15459281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh132
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