Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase

Chemical ligation has been used to alter motions in specific regions of dihydrofolate reductase from E. coli and to investigate the effects of localized motional changes on enzyme catalysis. Two isotopic hybrids were prepared; one with the mobile N‐terminal segment containing heavy isotopes ((2)H, (...

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Vydáno v:Angew Chem Int Ed Engl
Hlavní autoři: Luk, Louis Y. P., Ruiz‐Pernía, J. Javier, Adesina, Aduragbemi S., Loveridge, E. Joel, Tuñón, Iñaki, Moliner, Vincent, Allemann, Rudolf K.
Médium: Artigo
Jazyk:Inglês
Vydáno: WILEY‐VCH Verlag 2015
Témata:
Communications
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4985705/
https://ncbi.nlm.nih.gov/pubmed/26079622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201503968
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