Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase

Chemical ligation has been used to alter motions in specific regions of dihydrofolate reductase from E. coli and to investigate the effects of localized motional changes on enzyme catalysis. Two isotopic hybrids were prepared; one with the mobile N‐terminal segment containing heavy isotopes ((2)H, (...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Luk, Louis Y. P., Ruiz‐Pernía, J. Javier, Adesina, Aduragbemi S., Loveridge, E. Joel, Tuñón, Iñaki, Moliner, Vincent, Allemann, Rudolf K.
Formato: Artigo
Idioma:Inglês
Publicado em: WILEY‐VCH Verlag 2015
Assuntos:
Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4985705/
https://ncbi.nlm.nih.gov/pubmed/26079622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201503968
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