Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase

Chemical ligation has been used to alter motions in specific regions of dihydrofolate reductase from E. coli and to investigate the effects of localized motional changes on enzyme catalysis. Two isotopic hybrids were prepared; one with the mobile N‐terminal segment containing heavy isotopes ((2)H, (...

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Publicado en:Angew Chem Int Ed Engl
Autores principales: Luk, Louis Y. P., Ruiz‐Pernía, J. Javier, Adesina, Aduragbemi S., Loveridge, E. Joel, Tuñón, Iñaki, Moliner, Vincent, Allemann, Rudolf K.
Formato: Artigo
Lenguaje:Inglês
Publicado: WILEY‐VCH Verlag 2015
Materias:
Communications
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4985705/
https://ncbi.nlm.nih.gov/pubmed/26079622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201503968
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