Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase

Chemical ligation has been used to alter motions in specific regions of dihydrofolate reductase from E. coli and to investigate the effects of localized motional changes on enzyme catalysis. Two isotopic hybrids were prepared; one with the mobile N‐terminal segment containing heavy isotopes ((2)H, (...

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Dettagli Bibliografici
Pubblicato in:Angew Chem Int Ed Engl
Autori principali: Luk, Louis Y. P., Ruiz‐Pernía, J. Javier, Adesina, Aduragbemi S., Loveridge, E. Joel, Tuñón, Iñaki, Moliner, Vincent, Allemann, Rudolf K.
Natura: Artigo
Lingua:Inglês
Pubblicazione: WILEY‐VCH Verlag 2015
Soggetti:
Communications
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4985705/
https://ncbi.nlm.nih.gov/pubmed/26079622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201503968
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