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Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease
We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct sgRNA constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14 15 bp target sequences and MS2 binding loops, can activate gene expression using an active Cas9 nuclease, withou...
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| Publicado no: | Nat Biotechnol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4747789/ https://ncbi.nlm.nih.gov/pubmed/26436575 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3390 |
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