Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. We describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. We use these engineered Cas9 a...

Full description

Saved in:
Bibliographic Details
Published in:Nature
Main Authors: Konermann, Silvana, Brigham, Mark D., Trevino, Alexandro E., Joung, Julia, Abudayyeh, Omar O., Barcena, Clea, Hsu, Patrick D., Habib, Naomi, Gootenberg, Jonathan S., Nishimasu, Hiroshi, Nureki, Osamu, Zhang, Feng
Format: Artigo
Language:Inglês
Published: 2014
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4420636/
https://ncbi.nlm.nih.gov/pubmed/25494202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature14136
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items