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Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeats)...
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| Publicado no: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5526071/ https://ncbi.nlm.nih.gov/pubmed/28333914 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.016 |
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