Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeats)...

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Bibliografski detalji
Izdano u:Nat Protoc
Glavni autori: Joung, Julia, Konermann, Silvana, Gootenberg, Jonathan S., Abudayyeh, Omar O., Platt, Randall J., Brigham, Mark D., Sanjana, Neville E., Zhang, Feng
Format: Artigo
Jezik:Inglês
Izdano: 2017
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Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5526071/
https://ncbi.nlm.nih.gov/pubmed/28333914
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.016
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