A simple nonradioactive method for the determination of the binding affinities of antibodies induced by hapten bioconjugates for drugs of abuse

The accurate analytical measurement of binding affinities of polyclonal antibody in sera to heroin, 6-acetylmorphine (6-AM), and morphine has been a challenging task. A simple nonradioactive method that uses deuterium-labeled drug tracers and equilibrium dialysis (ED) combined with ultra performance...

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Udgivet i:Anal Bioanal Chem
Main Authors: Torres, Oscar B., Antoline, Joshua F. G., Li, Fuying, Jalah, Rashmi, Jacobson, Arthur E., Rice, Kenner C., Alving, Carl R., Matyas, Gary R.
Format: Artigo
Sprog:Inglês
Udgivet: Springer Berlin Heidelberg 2015
Fag:
Research Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4718952/
https://ncbi.nlm.nih.gov/pubmed/26677020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-015-9223-z
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