A rapid solution-based method for determining the affinity of heroin hapten-induced antibodies to heroin, its metabolites, and other opioids

We describe for the first time a method that utilizes microscale thermophoresis (MST) technology to determine polyclonal antibody affinities to small molecules. Using a novel type of heterologous MST, we have accurately measured a solution-based binding affinity of serum antibodies to heroin which w...

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Detalhes bibliográficos
Publicado no:Anal Bioanal Chem
Main Authors: Torres, Oscar B., Duval, Alexander J., Sulima, Agnieszka, Antoline, Joshua F. G., Jacobson, Arthur E., Rice, Kenner C., Alving, Carl R., Matyas, Gary R.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2018
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5956019/
https://ncbi.nlm.nih.gov/pubmed/29675707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-018-1060-4
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