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Synthetic CRISPR RNA-Cas9–guided genome editing in human cells

Genome editing with the clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 nuclease system is a powerful technology for manipulating genomes, including introduction of gene disruptions or corrections. Here we develop a chemically modified, 29-nucleotide synthetic CRISPR RNA (s...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Rahdar, Meghdad, McMahon, Moira A., Prakash, Thazha P., Swayze, Eric E., Bennett, C. Frank, Cleveland, Don W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4697396/
https://ncbi.nlm.nih.gov/pubmed/26589814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1520883112
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