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Directed evolution of the tryptophan synthase β-subunit for stand-alone function recapitulates allosteric activation
Enzymes in heteromeric, allosterically regulated complexes catalyze a rich array of chemical reactions. Separating the subunits of such complexes, however, often severely attenuates their catalytic activities, because they can no longer be activated by their protein partners. We used directed evolut...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2015
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4664345/ https://ncbi.nlm.nih.gov/pubmed/26553994 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1516401112 |
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