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Synthesis of β-branched tryptophan analogs using an engineered subunit of tryptophan synthase
We report that l-threonine may substitute for l-serine in the β-substitution reaction of an engineered subunit of tryptophan synthase from Pyrococcus furiosus, yielding (2S,3S)-β-methyltryptophan (β-MeTrp) in a single step. The trace activity of the wild-type β-subunit on this substrate was enhanced...
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| Publicado no: | J Am Chem Soc |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4948191/ https://ncbi.nlm.nih.gov/pubmed/27355405 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b04836 |
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