Chargement en cours...
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid libraries containing a randomized PAM as a functio...
Enregistré dans:
| Publié dans: | Genome Biol |
|---|---|
| Auteurs principaux: | , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2015
|
| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4653880/ https://ncbi.nlm.nih.gov/pubmed/26585795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0818-7 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|