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CRISPR/Cas9 searches for a protospacer adjacent motif by lateral diffusion

The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) nuclease has been widely applied in genetic engineering. Despite its importance in genome editing, aspects of the precise molecular mechanism of Cas9 activity remain ambiguous. In particular, because of the lack of a method with high spatio‐temporal re...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Globyte, Viktorija, Lee, Seung Hwan, Bae, Taegeun, Kim, Jin‐Soo, Joo, Chirlmin
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6376262/
https://ncbi.nlm.nih.gov/pubmed/30573670
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201899466
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