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CRISPR/Cas9 searches for a protospacer adjacent motif by lateral diffusion
The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) nuclease has been widely applied in genetic engineering. Despite its importance in genome editing, aspects of the precise molecular mechanism of Cas9 activity remain ambiguous. In particular, because of the lack of a method with high spatio‐temporal re...
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| Veröffentlicht in: | EMBO J |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
John Wiley and Sons Inc.
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6376262/ https://ncbi.nlm.nih.gov/pubmed/30573670 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201899466 |
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