CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif

Designing efficient and specific CRISPR single-guide RNAs (sgRNAs) is vital for the successful application of CRISPR technology. Currently, a growing number of new RNA-guided endonucleases with a different protospacer adjacent motif (PAM) have been discovered, suggesting the necessity to develop a v...

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Bibliografiske detaljer
Udgivet i:Int J Biol Sci
Main Authors: Zhao, Changzhi, Zheng, Xiaoguo, Qu, Wubin, Li, Guanglei, Li, Xinyun, Miao, Yi-Liang, Han, Xiaosong, Liu, Xiangdong, Li, Zhenhua, Ma, Yunlong, Shao, Qianzhi, Li, Haiwei, Sun, Fei, Xie, Shengsong, Zhao, Shuhong
Format: Artigo
Sprog:Inglês
Udgivet: Ivyspring International Publisher 2017
Fag:
Research Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5723913/
https://ncbi.nlm.nih.gov/pubmed/29230095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7150/ijbs.21312
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