Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid libraries containing a randomized PAM as a functio...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Genome Biol
Päätekijät: Karvelis, Tautvydas, Gasiunas, Giedrius, Young, Joshua, Bigelyte, Greta, Silanskas, Arunas, Cigan, Mark, Siksnys, Virginijus
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: BioMed Central 2015
Aiheet:
Method
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4653880/
https://ncbi.nlm.nih.gov/pubmed/26585795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0818-7
Tagit: Lisää tagi
Ei tageja, Lisää ensimmäinen tagi!

Samankaltaisia teoksia