Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid libraries containing a randomized PAM as a functio...

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Detalles Bibliográficos
Publicado en:Genome Biol
Main Authors: Karvelis, Tautvydas, Gasiunas, Giedrius, Young, Joshua, Bigelyte, Greta, Silanskas, Arunas, Cigan, Mark, Siksnys, Virginijus
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2015
Assuntos:
Method
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4653880/
https://ncbi.nlm.nih.gov/pubmed/26585795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0818-7
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