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Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid libraries containing a randomized PAM as a functio...
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| Publicado no: | Genome Biol |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4653880/ https://ncbi.nlm.nih.gov/pubmed/26585795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0818-7 |
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