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Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid libraries containing a randomized PAM as a functio...
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| 出版年: | Genome Biol |
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| 主要な著者: | , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
BioMed Central
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4653880/ https://ncbi.nlm.nih.gov/pubmed/26585795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0818-7 |
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