A versatile modular vector system for rapid combinatorial mammalian genetics

Here, we describe the multiple lentiviral expression (MuLE) system that allows multiple genetic alterations to be introduced simultaneously into mammalian cells. We created a toolbox of MuLE vectors that constitute a flexible, modular system for the rapid engineering of complex polycistronic lentivi...

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Enregistré dans:
Détails bibliographiques
Publié dans:J Clin Invest
Auteurs principaux: Albers, Joachim, Danzer, Claudia, Rechsteiner, Markus, Lehmann, Holger, Brandt, Laura P., Hejhal, Tomas, Catalano, Antonella, Busenhart, Philipp, Gonçalves, Ana Filipa, Brandt, Simone, Bode, Peter K., Bode-Lesniewska, Beata, Wild, Peter J., Frew, Ian J.
Format: Artigo
Langue:Inglês
Publié: American Society for Clinical Investigation 2015
Sujets:
Technical Advance
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4396471/
https://ncbi.nlm.nih.gov/pubmed/25751063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1172/JCI79743
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