A versatile modular vector system for rapid combinatorial mammalian genetics

Here, we describe the multiple lentiviral expression (MuLE) system that allows multiple genetic alterations to be introduced simultaneously into mammalian cells. We created a toolbox of MuLE vectors that constitute a flexible, modular system for the rapid engineering of complex polycistronic lentivi...

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發表在:J Clin Invest
Main Authors: Albers, Joachim, Danzer, Claudia, Rechsteiner, Markus, Lehmann, Holger, Brandt, Laura P., Hejhal, Tomas, Catalano, Antonella, Busenhart, Philipp, Gonçalves, Ana Filipa, Brandt, Simone, Bode, Peter K., Bode-Lesniewska, Beata, Wild, Peter J., Frew, Ian J.
格式: Artigo
語言:Inglês
出版: American Society for Clinical Investigation 2015
主題:
Technical Advance
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4396471/
https://ncbi.nlm.nih.gov/pubmed/25751063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1172/JCI79743
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