A versatile modular vector system for rapid combinatorial mammalian genetics

Here, we describe the multiple lentiviral expression (MuLE) system that allows multiple genetic alterations to be introduced simultaneously into mammalian cells. We created a toolbox of MuLE vectors that constitute a flexible, modular system for the rapid engineering of complex polycistronic lentivi...

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Detalhes bibliográficos
Publicado no:J Clin Invest
Main Authors: Albers, Joachim, Danzer, Claudia, Rechsteiner, Markus, Lehmann, Holger, Brandt, Laura P., Hejhal, Tomas, Catalano, Antonella, Busenhart, Philipp, Gonçalves, Ana Filipa, Brandt, Simone, Bode, Peter K., Bode-Lesniewska, Beata, Wild, Peter J., Frew, Ian J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Clinical Investigation 2015
Assuntos:
Technical Advance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4396471/
https://ncbi.nlm.nih.gov/pubmed/25751063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1172/JCI79743
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