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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

S-Adenosyl-l-methionine (AdoMet or SAM)-dependent methyltransferases (MTase) catalyze the transfer of the activated methyl group from AdoMet to specific positions in DNA, RNA, proteins and small biomolecules. This natural methylation reaction can be expanded to a wide variety of alkylation reactions...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Hanz, Gisela Maria, Jung, Britta, Giesbertz, Anna, Juhasz, Matyas, Weinhold, Elmar
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354249/
https://ncbi.nlm.nih.gov/pubmed/25490674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52014
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