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Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling

This work identifies the combination of enzymatic transfer and click labeling as an efficient method for the site-specific tagging of RNA molecules for biophysical studies. A double-activated analog of the ubiquitous co-substrate S-adenosyl-l-methionine was employed to enzymatically transfer a five...

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Bibliografski detalji
Glavni autori: Motorin, Yuri, Burhenne, Jürgen, Teimer, Roman, Koynov, Kaloian, Willnow, Sophie, Weinhold, Elmar, Helm, Mark
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3061074/
https://ncbi.nlm.nih.gov/pubmed/21037259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq825
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