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Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling
This work identifies the combination of enzymatic transfer and click labeling as an efficient method for the site-specific tagging of RNA molecules for biophysical studies. A double-activated analog of the ubiquitous co-substrate S-adenosyl-l-methionine was employed to enzymatically transfer a five...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3061074/ https://ncbi.nlm.nih.gov/pubmed/21037259 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq825 |
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