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A multifunctional bioconjugate module for versatile photoaffinity labeling and click chemistry of RNA

A multifunctional reagent based on a coumarin scaffold was developed for derivatization of naive RNA. The alkylating agent N3BC [7-azido-4-(bromomethyl)coumarin], obtained by Pechmann condensation, is selective for uridine. N3BC and its RNA conjugates are pre-fluorophores which permits controlled mo...

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Hlavní autoři: Kellner, Stefanie, Seidu-Larry, Salifu, Burhenne, Jürgen, Motorin, Yuri, Helm, Mark
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3167637/
https://ncbi.nlm.nih.gov/pubmed/21646334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr449
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