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A multifunctional bioconjugate module for versatile photoaffinity labeling and click chemistry of RNA
A multifunctional reagent based on a coumarin scaffold was developed for derivatization of naive RNA. The alkylating agent N3BC [7-azido-4-(bromomethyl)coumarin], obtained by Pechmann condensation, is selective for uridine. N3BC and its RNA conjugates are pre-fluorophores which permits controlled mo...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3167637/ https://ncbi.nlm.nih.gov/pubmed/21646334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr449 |
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