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Massively parallel determination and modeling of endonuclease substrate specificity
We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity screening in a bacterial selection system. We characterized the substrate specificity and kinetics of these endonucle...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4267613/ https://ncbi.nlm.nih.gov/pubmed/25389263 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku1096 |
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