Massively parallel determination and modeling of endonuclease substrate specificity

We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity screening in a bacterial selection system. We characterized the substrate specificity and kinetics of these endonucle...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Thyme, Summer B., Song, Yifan, Brunette, T. J., Szeto, Mindy D., Kusak, Lara, Bradley, Philip, Baker, David
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2014
Témata:
Nucleic Acid Enzymes
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4267613/
https://ncbi.nlm.nih.gov/pubmed/25389263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku1096
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