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Massively parallel characterization of restriction endonucleases

Restriction endonucleases are highly specific in recognizing the particular DNA sequence they act on. However, their activity is affected by sequence context, enzyme concentration and buffer composition. Changes in these factors may lead to either ineffective cleavage at the cognate restriction site...

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Detalhes bibliográficos
Main Authors: Kamps-Hughes, Nick, Quimby, Aine, Zhu, Zhenyu, Johnson, Eric A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3675476/
https://ncbi.nlm.nih.gov/pubmed/23605040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt257
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