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Massively parallel characterization of restriction endonucleases
Restriction endonucleases are highly specific in recognizing the particular DNA sequence they act on. However, their activity is affected by sequence context, enzyme concentration and buffer composition. Changes in these factors may lead to either ineffective cleavage at the cognate restriction site...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3675476/ https://ncbi.nlm.nih.gov/pubmed/23605040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt257 |
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