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Implementation of the CRISPR-Cas9 system in fission yeast
Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of RNA (rrk1) and a ribozyme to produce the ta...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4215166/ https://ncbi.nlm.nih.gov/pubmed/25352017 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms6344 |
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