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Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe
Controllable and reversible transcriptional repression is an essential method to study gene functions. A systematic knock-down method using catalytically inactive Cas9 (dCas9) was originally established in bacteria. dCas9 forms a ribonucleoprotein with a small guide RNA and uses it to recognize a sp...
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| Publié dans: | G3 (Bethesda) |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8137136/ https://ncbi.nlm.nih.gov/pubmed/33617628 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/g3journal/jkab051 |
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