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SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast
The CRISPR/Cas9 system allows scarless, marker-free genome editing. Current CRISPR/Cas9 systems for the fission yeast Schizosaccharomyces pombe rely on tedious and time-consuming cloning procedures to introduce a specific sgRNA target sequence into a Cas9-expressing plasmid. In addition, Cas9 endon...
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| Publicado no: | Wellcome Open Res |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
F1000 Research Limited
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7721064/ https://ncbi.nlm.nih.gov/pubmed/33313420 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.16405.1 |
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