SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast

The CRISPR/Cas9 system allows scarless, marker-free genome editing. Current CRISPR/Cas9 systems for the fission yeast  Schizosaccharomyces pombe rely on tedious and time-consuming cloning procedures to introduce a specific sgRNA target sequence into a Cas9-expressing plasmid. In addition, Cas9 endon...

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Bibliografiske detaljer
Udgivet i:Wellcome Open Res
Main Authors: Torres-Garcia, Sito, Di Pompeo, Lorenza, Eivers, Luke, Gaborieau, Baptiste, White, Sharon A., Pidoux, Alison L., Kanigowska, Paulina, Yaseen, Imtiyaz, Cai, Yizhi, Allshire, Robin C.
Format: Artigo
Sprog:Inglês
Udgivet: F1000 Research Limited 2020
Fag:
Method Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7721064/
https://ncbi.nlm.nih.gov/pubmed/33313420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.16405.1
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