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Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast

The CRISPR/Cas9 system enables the editing of genomes of numerous organisms through the induction of the double-strand breaks (DSB) at specific chromosomal targets. We improved the CRISPR/Cas9 system to ease the direct introduction of a point mutation or a tagging sequence into the chromosome by com...

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Detalhes bibliográficos
Publicado no:G3 (Bethesda)
Main Authors: Hayashi, Aki, Tanaka, Katsunori
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6469419/
https://ncbi.nlm.nih.gov/pubmed/30755408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.118.200976
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