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Synthesis of thioester peptides for the incorporation of thioamides into proteins by native chemical ligation()
Thioamides can be used as photoswitches, as reporters of local environment, as inhibitors of enzymes, and as fluorescence quenchers. We have recently demonstrated the incorporation of thioamides into polypeptides and proteins using native chemical ligation (NCL). In this protocol, we describe proced...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4199922/ https://ncbi.nlm.nih.gov/pubmed/24408658 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/psc.2589 |
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