Labeling Proteins with Fluorophore/Thioamide FRET Pairs by Combining Unnatural Amino Acid Mutagenesis and Native Chemical Ligation

We have recently shown that p-cyanophenylalanine (Cnf) and a thioamide can be used as a minimally-perturbing Förster resonant energy transfer (FRET) pair to monitor protein conformation. We have also shown that thioamide analogs of natural amino acids can be incorporated into full-sized proteins thr...

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Autores principales: Wissner, Rebecca F., Batjargal, Solongo, Fadzen, Colin M., Petersson, E. James
Formato: Artigo
Lenguaje:Inglês
Publicado: 2013
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3721677/
https://ncbi.nlm.nih.gov/pubmed/23594264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja4005943
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