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Synthesis of thioester peptides for the incorporation of thioamides into proteins by native chemical ligation()
Thioamides can be used as photoswitches, as reporters of local environment, as inhibitors of enzymes, and as fluorescence quenchers. We have recently demonstrated the incorporation of thioamides into polypeptides and proteins using native chemical ligation (NCL). In this protocol, we describe proced...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4199922/ https://ncbi.nlm.nih.gov/pubmed/24408658 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/psc.2589 |
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