Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA, at 2.5 Å resolution. The structure revealed a bilobed architecture composed o...

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Hlavní autoři: Nishimasu, Hiroshi, Ran, F. Ann, Hsu, Patrick D., Konermann, Silvana, Shehata, Soraya, Dohmae, Naoshi, Ishitani, Ryuichiro, Zhang, Feng, Nureki, Osamu
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4139937/
https://ncbi.nlm.nih.gov/pubmed/24529477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2014.02.001
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