Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA, at 2.5 Å resolution. The structure revealed a bilobed architecture composed o...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Nishimasu, Hiroshi, Ran, F. Ann, Hsu, Patrick D., Konermann, Silvana, Shehata, Soraya, Dohmae, Naoshi, Ishitani, Ryuichiro, Zhang, Feng, Nureki, Osamu
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2014
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4139937/
https://ncbi.nlm.nih.gov/pubmed/24529477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2014.02.001
Etiketak: Etiketa erantsi
Etiketarik gabe, Izan zaitez lehena erregistro honi etiketa jartzen!

Antzeko izenburuak