Llwytho...

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA, at 2.5 Å resolution. The structure revealed a bilobed architecture composed o...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Nishimasu, Hiroshi, Ran, F. Ann, Hsu, Patrick D., Konermann, Silvana, Shehata, Soraya, Dohmae, Naoshi, Ishitani, Ryuichiro, Zhang, Feng, Nureki, Osamu
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2014
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4139937/
https://ncbi.nlm.nih.gov/pubmed/24529477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2014.02.001
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