Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA, at 2.5 Å resolution. The structure revealed a bilobed architecture composed o...

Descripció completa

Guardat en:
Dades bibliogràfiques
Autors principals: Nishimasu, Hiroshi, Ran, F. Ann, Hsu, Patrick D., Konermann, Silvana, Shehata, Soraya, Dohmae, Naoshi, Ishitani, Ryuichiro, Zhang, Feng, Nureki, Osamu
Format: Artigo
Idioma:Inglês
Publicat: 2014
Matèries:
Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4139937/
https://ncbi.nlm.nih.gov/pubmed/24529477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2014.02.001
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars