Identification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry

We report a methodology that combines affinity acetylation with MS analysis for accurate mapping of an inhibitor-binding site to a target protein. For this purpose, we used a known HIV-1 integrase inhibitor containing aryl di-O-acetyl groups (Acetylated-Inhibitor). In addition, we designed a control...

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Bibliografiska uppgifter
Huvudupphovsmän: Shkriabai, Nick, Patil, Sachindra S., Hess, Sonja, Budihas, Scott R., Craigie, Robert, Burke, Terrence R., Le Grice, Stuart F. J., Kvaratskhelia, Mamuka
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2004
Ämnen:
Biological Sciences
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC406438/
https://ncbi.nlm.nih.gov/pubmed/15118107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0400873101
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